Mystery fiber update

A quick update on our mystery fiber (see my previous post for details):

Today I decided to do a chemical spot-test to see if I could determine if the fiber was cellulose or protein-based. Chemical spot tests are inexpensive, generally simple procedures that conservators may use to characterize materials on artifacts. These spot tests are often carried out on small samples removed from artifacts using chemical reagents. In the case of my mystery fiber, I cut a small piece off of one of the fiber samples I previously examined under the microscope-this small piece was enough for a spot test, and there was no need to remove more material from the coffin in order to do this.

The first test I chose to carry out was the Biuret test for protein (according to instructions in Material Characterization Tests for Objects of Art and Archaeology), using copper(II) sulfate. After placing a drop of copper (II) sulfate solution on the sample, I waited for a few minutes, then soaked up the excess solution and added a drop of sodium hydroxide solution to the sample. It immediately turned purple (see below), which indicates the presence of protein (and just in case it’s not clear on your screen, believe me, it is purple!).

Magnified image of the sample used for the protein spot test. The purple hue indicates a positive reaction for presence of protein. 50X magnification

What this means is still unclear, but it’s another clue. It is possible that my earlier comparison of this fiber to sinew was not a bad suggestion! But it’s also possible that this fiber was coated in a protein-based glue before it was incorporated into the gesso (or something like this).

This calls for further investigation!